Neonatal Transplantation Confers Maturation of PSC-Derived Cardiomyocytes Conducive to Modeling Cardiomyopathy
Neonatal Transplantation Confers Maturation of PSC-Derived Cardiomyocytes Conducive to Modeling Cardiomyopathy
Blog Article
Summary: Pluripotent fleshlight automatique stem cells (PSCs) offer unprecedented opportunities for disease modeling and personalized medicine.However, PSC-derived cells exhibit fetal-like characteristics and remain immature in a dish.This has emerged as a major obstacle for their application for late-onset diseases.
We previously showed that there is a neonatal arrest of long-term cultured PSC-derived cardiomyocytes (PSC-CMs).Here, we demonstrate that PSC-CMs mature into adult CMs when transplanted into neonatal hearts.PSC-CMs became similar to adult CMs in morphology, structure, and function within a month of transplantation into rats.
The similarity was further supported by single-cell RNA-sequencing analysis.Moreover, this in vivo maturation allowed patient-derived PSC-CMs to reveal the disease phenotype of arrhythmogenic right ventricular cardiomyopathy, which manifests predominantly in adults.This study lays a foundation for understanding human CM maturation and pathogenesis and can be instrumental in PSC-based modeling 100w products of adult heart diseases.
: Pluripotent stem cell (PSC)-derived cells remain fetal like, and this has become a major impediment to modeling adult diseases.Cho et al.find that PSC-derived cardiomyocytes mature into adult cardiomyocytes when transplanted into neonatal rat hearts.
This method can serve as a tool to understand maturation and pathogenesis in human cardiomyocytes.Keywords: cardiomyocyte, maturation, iPS, cardiac progenitor, neonatal, disease modeling, cardiomyopathy, ARVC, T-tubule, calcium transient, sarcomere shortening.